首页期刊信息编委及顾问期刊发行联系方式使用帮助常见问题ENGLISH
位置:首页 >> 正文
腐蚀法制备碳化硅量子点标记材料及其光学性能
作者:康杰 宋月鹏 高东升 朱彦敏 毛志泉 孙祥鸣 尹承苗 贾浩 KIMHyoungseop 
单位:1. 山东农业大学机械与电子工程学院 山东 泰安 271018 2. 山东省园艺机械与装备重点实验室 山东 泰安 271018 3. 山东农业大学园艺学院 山东 泰安 271018 4. 浦项工科大学材料科学与工程学院 韩国 浦项 790784 
关键词:化学腐蚀 碳化硅量子点 光致发光 活体细胞标记 出芽短梗霉菌 
分类号:O482;O614
出版年,卷(期):页码:2013,41(12):1709-1714
DOI:
摘要:
以硝酸和氢氟酸为腐蚀液对自蔓延燃烧合成的纳米均质碳化硅颗粒进行腐蚀,而后进行超声空化破碎分散及高速离心处理,获得无细胞毒性的碳化硅量子点标记材料,对其微观结构的演变过程及光学性能进行检测,对出芽短梗霉菌(Aureobasidium pulluans)活体细胞进行标记并长时程荧光成像。结果表明,自蔓延合成的SiC粉体颗粒极易腐蚀成网格状镂空结构,超声空化破碎及高速离心层析剪裁后获得尺寸高度单分散的碳化硅量子点(约为1~2.5 nm),小于体材料的激子Bohr直径(5.4 nm),会产生强烈的光致发光效应。活体细胞标记及荧光成像结果表明,腐蚀法制备出的碳化硅量子点具有较高的生物相容性,同时对其标记及长时程荧光成像原理进行了初步探讨。
open reasons why married men cheat My husband cheated on me
printable coupons for cialis drug discount coupons coupon for free cialis
Silicon carbide nanoparticles fabricated via self-propagating high-temperature synthesis (SHS) were etched in the mixture solution of nitric and hydrofluoric acid. The non-cytotoxic labeling material of silicon carbide quantum dots (QDs) were prepared by ultrasonic cavitation and high-speed centrifugation. The microstructure and optical performance of SiC QDs were analyzed. The living cell of Aureobasidium pulluans was labeled and observed by long-term-distance fluorescence imaging analysis. The results show that the SiC particles prepared by SHS are formed as a grid-like hollow structure in the mixture acid solution. The SiC QDs in the size range from 1 to 2.5 nm were fabricated by cavitation crushing with ultrasound and chromatography with high-speed centrifugal force, leading to the size of less than the corresponding exciton Bohr diameter (5.4 nm) for the intensive photoluminescence. Moreover, the SiC QDs prepared have a superior biocompatibility. In addition, the mechanism on the labeling and long-term-distance fluorescence imaging analysis of the living cells was also discussed.
how to cheat signs of a cheater wife cheat story
read wifes cheat reasons wives cheat on their husbands
基金项目:
国家自然科学基金项目(51001111);“十二五”国家支撑计划项目(2011BAD12B02);山东省博士后创新基金项目(201203101);中国博士后科学基金项目(2013M531636)资助。
作者简介:
第一作者:康 杰(1985—),男,硕士研究生。 通信作者:宋月鹏(1971—),男,博士,副教授。
abortion las vegas period after abortion abortion pictures
amoxicillin amoxicillin amoxicillin
sumatriptan side effects sumatriptan side effects sumatriptan side effects
参考文献:
[1] MICHALET X, PINAUD F F, BWNTOLILA L A, et al. Quantum dots for live cells, in vivo imaging, and diagnostics [J]. Science, 2005, 307: 538–544. [2] GAO X H, CUI Y Y, LEVENSON R M, et al. In vivo cancer targeting and imaging with semiconductor quantum dots [J]. Nat Biotechnol, 2004, 22(8): 969–976. [3] 王德平, 胡朗, 吴立功, 等. CdSe/SiO2荧光微球的制备及其荧光性能[J]. 硅酸盐学报. 2010, 38(10): 2001–2004. WANG Deping, HU Lang, WU Ligong, et al. J Chin Ceram Soc, 2010, 38(10): 2001–2004. [4] MEDINTZ I L, UYEDA H T, GOLDMAN E R, et al. Quantum dot bioconjugates for imaging, labeling and sensing [J]. Nature, 2005, 4(6): 435–446. [5] 吴立功, 王德平, 姚爱华, 等. 水热法制备细胞标记用CdSe半导体量子点材料[J]. 硅酸盐学报, 2009, 37(9): 1580–1584. WU Ligong, WANG Deping, YAO Aihua, et al. J Chin Ceram Soc, 2009, 37(9): 1580–1584. [6] 王璐, 王德平, 黄文旵, 等. 水相制备硒化镉半导体量子点的荧光性能[J]. 硅酸盐学报, 2005, 33(10): 1224–1230. WNAG Lu, WANG Deping, HUANG Wenchan, et al. J Chin Ceram Soc, 2005, 33(10): 1224–1230. [7] HEN N, HE Y, SU Y, et al. The cytotoxicity of cadmium-based quantum dots [J]. Biomaterials, 2012, 33(5): 1238–1244. [8] 孙祥鸣, 宋月鹏, 高东升, 等. 碳化硅量子点制备新工艺及其活体细胞荧光成像[J]. 农业工程学报, 2012, 28(24): 260–264. SUN Xiangming, SONG Yuepeng, GAO Dongsheng, et al. Trans CSAE (in Chinese), 2012, 28(24): 260–264. [9] BOTSOA J, LYSENKO V, GELOEN A, et al. Application of 3C-SiC quantum dots for living cell imaging [J]. Appl Phys Lett, 2008, 92: 173902 (1–3). [10] FAN J Y, LI H X, JIANG J, et al. 3C-SiC nanocrystals as fluorescent biological labels [J]. Small, 2008, 4(8): 1058–1062. [11] FAN J Y, WU X L, CHU P K. Low-dimensional SiC nanostructures: fabrication, luminescence, and electrical properties [J]. Prog Mater Sci, 2006, 51: 983–1031. [12] BLUET J M, BOTSOA J, ZAKHARKO Y, et al. Chapter 11-SiC as a biocompatible marker for cell labeling [A]// SADDOW S E, ed. Silicon Carbide Biotechnology: A Biocompatible Semiconductor for Advanced Biomedical Devices and Applications [M]. Oxford UK: Elsevier’s Science and Technology Rights Department, 2012. [13] 刘爱平, 王琦琛, 郭振, 等. 细胞生物学荧光技术原理和应用[M]. 合肥: 中国科学技术大学出版社, 2007: 2. [14] ZHU J, LIU Z, WU X L, et al. Luminescent small-diameter 3C-SiC nanocrystals fabricated via a simple chemical etching method [J]. Nanotechnology, 2007, 18: 365603(1–5). [15] 蔡海荣, 陈大英, 李江涛. 化学激励燃烧合成SiC超细粉末的工艺和机理研究[J]. 硅酸盐学报, 2002, 30(3): 393–397. CAI Hairong, CHEN Daying, LI Jiangtao. J Chin Ceram Soc, 2002, 30(3): 393–397. [16] 张婵, 郑爽英. 超声空化效应及其应用[J]. 水资源与水工程学报, 2009, 20(1): 136–138. ZHANG Chan, ZHENG Shuangying. J Water Resour Water Eng (in Chinese), 2009, 20(1): 136–138. [17] 范吉阳, 吴兴龙, 邱腾. 3C-SiC纳米颗粒量子限制效应的实验证据[J]. 物理, 2005, 34(8): 570–572. FAN JiYang, WU XingLong, QIU Teng. Physics (in Chinese), 2005, 34(8): 570–572. [18] http://fiber.ofweek.com/2009-08/ART-210001-8110-28417313.html [EB/OL], 2012–04–26. [19] 郁美娟, 刘维学, 王德平, 等. 巯基乙酸稳定的CdSe/ZnS核壳结构量子点的制备与表征[J]. 硅酸盐学报, 2007, 35(7): 822–827. YU Meijuan, LIU Weixue, WANG Deping, et al. J Chin Ceram Soc, 2007, 35(7): 822–827. [20] 邱军付, 杨筠, 李江涛. 表面预处理对燃烧合成β-SiC粉体水基浆料流动性的影响[J]. 硅酸盐学报, 2010, 38(4): 597–601. QIU Junfu, YANG Yun, LI Jiangtao. J Chin Ceram Soc, 2010, 38(4): 597–601. [21] 王雁, 邹建中. 超声空化的升学效应机制及其应用[J]. 中华物理医学与康复杂志, 2011, 33(3): 224–226. WANG Yan, ZOU Jianzhong. Chin J Phys Med Rehabil (in Chinese), 2011, 33(3): 224–226. [22] SAHU T, GHOSH B, PRDHAN S K, et al. Diverse role of silicon carbide in the domain of nanomaterials [J]. Int J Electrochem, 2012, doi:10.1155/2012/271285. [23] LI Y, CHEN C X, LI J T, et al. Surface charges and optical characteristic of colloidal cubic SiC nanocrystals [J]. Nanoscale Res Lett, 2011, 6: 454–461. [24] GORDON W L. Distribution and prevalence of fusarium moniliforme sheld [J]. Nature, 1960, 186: 698–700.
redirect husband cheated unfaithful husband
open open My husband cheated on me
amoxicillin amoxicillin amoxicillin
服务与反馈:
文章下载】【加入收藏
中国硅酸盐学会《硅酸盐学报》编辑室
京ICP备10016537号-2
京公网安备 11010802024188号
地址:北京市海淀区三里河路11号    邮政编码:100831
电话:010-57811253  57811254    
E-mail:jccs@ceramsoc.com