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微生物水泥矿化过程受环境中生物的影响
作者: 鹏1 2 邵光辉1 2 赵志峰1 2 黄容聘1 2 
单位:1. 南京林业大学土木学院 南京 210037 2. 江苏省水土保持与生态修复重点实验室 南京 210037 
关键词:微生物水泥 方解石 微生物 矿化 
分类号:TU522.07
出版年,卷(期):页码:2017,45(11):0-0
DOI:
摘要:

 分别从光密度(OD 值)、pH 值、Ca2+浓度和无机碳含量(DIC)4 个方面对环境中生物对微生物水泥矿化过程的影响机理展开研究。结果表明:虽然环境中的微生物能为CaCO3 晶体的沉积提供额外的成核位置,但其自身的生长繁殖、呼吸作用会消耗大量的营养物质,导致试样中pH 值和CO32–浓度的降低,不利于碳酸钙沉积的析出。造成在工程应用中需要生成大体量的CaCO3 时,速率、效率明显低于无环境中生物时的情形。因此,在微生物水泥技术实际应用中,必须考虑环境中已有生物的影响。试样中仅加入产脲酶菌就可以达到持续生成CaCO3 的目的,建议不加入营养物质以降低刺激环境中生物非矿化反应的风险。

 The effect of indigenous microorganisms on the biomineralization of Microbe Cement was investigated via the measurements of optical density and pH value as well as the evolution of dissolved calcium and dissolved inorganic carbon, respectively. The results show that the dissolved calcium is precipitated as CaCO3 reacts quickly in the bacteria-free culture rather than in the nature, despite the presence of the indigenous microorganisms can serve as the nucleation sites in the form of bacterial cells. The indigenous microorganisms exhibit a considerably high growth rate, resulting in less favorable chemical conditions in the nature culture, i.e., lower pH value and lower CO3 2- concentration. It is suggested that the presence of the indigenous microorganisms could decelerate the biomineralization of Microbe Cement and their effect should be taken into consideration. Furthermore, the injection of S. pasteurii does not require the addition of any nutrients for ureolysis to proceed over several days. The absence of nutrient addition reduces the risk of stimulating metabolic process other than ureolysis.

基金项目:
国家自然科学基金(51578293);江苏省高校自然科学研究面上项目(15KJB580008)。
作者简介:
刘鹏(1985–),男,博士,讲师。
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